Reading Code 128B In .NET Framework
Using Barcode Control SDK for VS .NET Control to generate, create, read, scan barcode image in .NET framework applications.
TABLE 15.8. Data Used to Search for a Peptide Sequence Using the Tag Approacha Match Criteria Enzyme speci city Measured peptide molecular mass Run of sequence ions Type of ion series Partial sequence Mass of region 1 (m1) Mass of region 3 (m3) Search string Trypsin 2111 0.4 977.4, 1074.5, 1161.5 b series (see Fig. 15.13) PS 977.4 949.5 (977.4)PS(949.5)
Print Code128 In .NET
Using Barcode maker for .NET framework Control to generate, create Code128 image in Visual Studio .NET applications.
a The masses are monoisotopic and in daltons. The mass difference between the 997.4 and 1074.5 peaks corresponds to the mass of a proline residue.
Scanning Code 128A In VS .NET
Using Barcode reader for .NET framework Control to read, scan read, scan image in VS .NET applications.
15.6. PROTEIN PEPTIDE SEQUENCING There are several instrumental MS methods that can be used to obtain sequence information from proteins and peptides. ESI-triple-quadrupole is frequently used; this con guration produces a reasonable number of fragments, the resolution is usually suf cient and the equipment is relatively inexpensive. With this MS/MS con guration, peptides up to 2500 Da can be analyzed. A MALDI with a TOF re ectron and Fourier transform ion cyclotron resonance mass analyzers are also beginning to be used.21 A typical procedure for protein sequencing begins by digestion with a protease such as trypsin in order to obtain a collection of tryptic peptides. Other enzymes and their cleavage sites are described elsewhere.22 This peptide collection can be ionized using ESI, separated by the rst quadrupole according to m/z ratios, and then fragmented (CID), with the resulting fragments analyzed in the third quadrupole. The mass spectrum can be analyzed to establish the peptide sequence, or compared directly with a peptide database. Figure 15.12 depicts this typical protocol. The CID procedure causes more or less random peptide bond cleavage. Therefore, a number of fragments are obtained that differ by a single amino acid residue. Several types of fragments are produced, and their nomenclature is shown in Figure 15.13. Two main classes of ions are formed by CID, those that contain the C terminus plus one or more additional residues (ions of types xn , yn, and zn ) and those that contain the N-terminus and one or more additional residues (ions of types an , bn , and cn ). Ions of types yn and bn are formed by the rupture of amide bonds, and the mass differences between these fragments are limited to the masses of the naturally occurring 19 different amino acid residues (Table 15.9). Isoleucine and leucine,
Bar Code Creation In VS .NET
Using Barcode encoder for VS .NET Control to generate, create barcode image in VS .NET applications.
Scanning Barcode In .NET
Using Barcode decoder for VS .NET Control to read, scan read, scan image in .NET applications.
Figure 15.12. Typical procedure used to obtain protein sequence information.
Code 128B Drawer In C#
Using Barcode creator for Visual Studio .NET Control to generate, create USS Code 128 image in .NET applications.
which are structural isomers and therefore have the same molecular weight, cannot be distinguished with MS. Moreover, lysine and glutamine can only be differentiated if resolution is high, since their mass difference is only 0.0432 Da. The sequencing process consists of the simultaneous measurement of a peptide collection, as shown in Figure 15.14. The sequence is obtained by observation of
Code 128A Generation In .NET Framework
Using Barcode drawer for ASP.NET Control to generate, create Code 128B image in ASP.NET applications.
Figure 15.13. Nomenclature used for the more common fragments produced by CID peptide fragmentation. The b and y fragment families , that occur when the peptide bond is broken, are normally produced at higher concentration.
Code 128 Code Set A Printer In VB.NET
Using Barcode creation for Visual Studio .NET Control to generate, create Code-128 image in VS .NET applications.
Barcode Printer In Visual Studio .NET
Using Barcode creation for Visual Studio .NET Control to generate, create bar code image in .NET applications.
TABLE 15.9. Average Masses of Amino Acids in Their Free and Residue Statesa Codes 3-Letter Ala Arg Asn Asp Cys Gln Glu Gly His Ile Leu Lys Met Phe Pro Ser Thr Trp Tyr Val Mr 1-Letter Residue Free A R N D C Q E G H I L K M F P S T W Y V 71.0786 156.1870 114.1036 115.0884 103.1386 128.1304 129.1152 57.0518 137.1408 113.1590 113.1590 128.1736 131.1922 147.1762 97.1164 87.0780 101.1048 186.2128 163.1756 99.1322 89.0938 174.2022 132.1188 133.1036 121.1538 146.1456 147.1304 75.0670 155.1560 131.1742 131.1742 146.1888 149.2074 165.1914 115.1316 105.0932 119.1200 204.2280 181.1908 117.1474
Paint DataMatrix In .NET Framework
Using Barcode creation for Visual Studio .NET Control to generate, create Data Matrix ECC200 image in VS .NET applications.
Amino Acid Alanine Arginine Asparagine Aspartic acid Cysteine Glutamine Glutamic acid Glycine Histidine Isoleucine Leucine Lysine Methionine Phenylalanine Proline Serine Threonine Tryptophan Tyrosine Valine
Code 3 Of 9 Creation In .NET
Using Barcode drawer for .NET framework Control to generate, create Code39 image in .NET framework applications.
a One letter codes are customary in the MS area. Reprinted, with permission, from M. Mann and M. Wilm, Anal. Chem. 66 (No 24), 1994, 4390-4399. Error Tolerant Identi cation of Peptides in Sequence Databases by Peptide Sequence Tags . Copyright # 1994 American Chemical Society.
Code 93 Full ASCII Maker In VS .NET
Using Barcode maker for .NET Control to generate, create ANSI/AIM Code 93 image in Visual Studio .NET applications.
the mass differences within one family of ions; the y family is fully represented in this example. The obtained sequence was then matched to an E. coli membrane protein.23 With this instrumentation, it is possible to analyze peptides with MW up to 2500 Da, although some limitations apply; if only a limited peptide collection is obtained, only limited sequence information can be produced. Sequence information can also be obtained using Edman degradation to remove amino-terminal residues from a peptide, to produce a collection of peptides. MALDI TOF can then be used to obtain the peptides masses, and the sequence determined by mass difference between consecutive peptides. This methodology is called protein ladder sequencing, and allows information to be obtained for up to 30 residues. This method is useful for the identi cation of posttranslational modi cations, such as phosphorylated amino acid residues.24 Once a peptide family has been sequenced, the next step is to overlap the available sequence information, in order to obtain the protein sequence. However, peptide sequences are more frequently used to identify proteins by searching the peptide sequences in databases. Several databases are available, and their utility
Creating Code-128 In C#.NET
Using Barcode maker for .NET Control to generate, create Code-128 image in .NET framework applications.
Code-39 Creation In Visual Basic .NET
Using Barcode printer for VS .NET Control to generate, create Code 39 image in .NET applications.
Barcode Encoder In Java
Using Barcode generator for Java Control to generate, create bar code image in Java applications.
Decode Bar Code In Java
Using Barcode scanner for Java Control to read, scan read, scan image in Java applications.
Barcode Maker In .NET
Using Barcode creation for ASP.NET Control to generate, create barcode image in ASP.NET applications.