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1. H.-W. Hsu, Separation by Centrifugal Phenomena , Techniques of Chemistry, Vol. XVI, John Wiley & Sons, Inc., New York, 1981, p. 111. 2. D. Rickwood and G. D. Birnie, in Centrifugal Separations in Molecular and Cell Biology. G. D. Birnie and D. Rickwood, Eds., Butterworths, London, 1978, pp. 1 3. 3. P. Sheeler, Centrifugation in Biology and Medical Science, John Wiley & Sons, Inc., New York, 1981, pp. 17 20. 4. V. Sitaramam and M. K. J. Sarma, Proc. Natl. Acad. Sci. U.S.A. 78, 1981, 3441 3445. 5. D. Rickwood, Ed., Centrifugation-A Practical Approach, IRL Press, Oxford, UK, 1984, pp. 295 304.
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6. P. Sheeler, Centrifugation in Biology and Medical Science, John Wiley & Sons, Inc., New York, 1981, pp 169 176. 7. C. A. Price. Centrifugation in Density Gradients, Academic Press, New York, 1982, p. 114. 8. C. A. Price. Centrifugation in Density Gradients, Academic Press, New York, 1982, pp. 114 126. 9. D. Rickwood, Ed., Centrifugation-A Practical Approach, IRL Press, Oxford, UK, 1984, pp. 168 189. 10. H.-W. Hsu, Separation by Centrifugal Phenomena , Techniques of Chemistry, Vol. XVI, John Wiley & Sons, Inc., New York, 1981, pp. 220 228. 11. P. Lavrenko, V. Lavrenko, and V. Tsvetkov, in Analitycal Ultracentrifugaton V, H. Colfen volume Ed., F. Kremer and G. Lagaly, Eds., Progress in Colloid and Polymer Science, Vol. 113, 1999, pp. 14 22. 12. R. Gauglitz, in Analitycal Ultracentrifugaton, J. Behlke guest Ed., F. Kremer and G. Lagaly, Eds., Progress in Colloid and Polymer Science, Vol. 99, 1995, pp.199 208. 13. P. Sheeler, Centrifugation in Biology and Medical Science. John Wiley & Sons, Inc., New York, 1981, pp. 23 25. 14. B. D. Young, in Centrifugation-A Practical Approach, D. Rickwood, Ed., IRL Press, Oxford, UK, 1984, p. 133. 15. H. K. Schachman, Ultracentrifugation in Biochemisty, Academic Press, New York, 1959, pp. 201 247. 16. V. J. Hindson, P. C. E. Moody, A. J. Rowe, and W. V. Shaw, J. Biol. Chem. 275, 2000, 461 466. 17. H. K. Schachman, Ultracentrifugation in Biochemisty. Academic Press, New York, 1959, p. 91. 18. A. M ller-Larsen and T. Christensen, J. Virol. Methods 73, 1998, 151 161. 19. J. M. Graham, J. A. Higgins, T. Gillott, T. Taylor, J. Wilkinson, T. Ford, and D. Billington, Atherosclerosis 124, 1996, 125 135.
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PROBLEMS 1. (a) Calculate the RCF maximum (RCFmax), minimum (RCFmin), and average (RCFav), for a centrifugation tube in which the meniscus is 12 cm from the rotation axis and the bottom at 22 cm. The rotor is driven at 27,000 rpm. (b) If the rotor is driven at half-velocity (13,500 rpm) will the g force applied to the solution be approximately one-half 2. (a) A titanium xed-angle rotor can be run at a maximum speed of 52,000 rpm. It has rmax 10.8 cm and rmin 3.2 cm. Calculate the k (clearing) value. (b) Compare the obtained value with the ones presented in Table 13.2. Choose the best rotor for the separation of soluble proteins from a mammalian cell homogenate. 3. A vertical rotor data sheet speci es k 304. The tubes for this rotor have an internal diameter of 2.4 cm, with rmin 13.6 cm. What is the maximum speed for this rotor (Note that in real situations, aging of the rotor and accumulation
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TABLE 13.2. Values of k and Total Capacity for Ultraspeed Rotorsa Rotor Type Swinging-bucket Swinging-bucket Swinging-bucket Fixed-angle (18 ) Fixed-angle (14 ) Fixed-angle (29 ) Vertical Vertical Vertical
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RCFmax (g) 90,300 285,000 484,200 59,200 94,500 511,000 70,000 240,600 510,000
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RCFmin (g) 39,300 119,000 254,000 29,600 63,300 220,800 50,400 173,400 416,600
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Total Capacity (mL) 102 84 26.4 940 210 112 312 280 40.8
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k 338 137 45 398 113 38 123 34 8
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