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%R 91 73 85 61 83 50 28 73 89 65 71 8 15 58 0 12 2 6 12 24 19 15 15 5 11 93 42 19 27 6
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%R 83 84 58 82 48 76 46 64 61 62 18 8 11 14 2 4 5 4 8 7 2 3 82 18 16 5 8
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a All Pe and SD(Pe ) are in units of 10 6 cm/s; (nd) compound not detected in the acceptor compartment
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Kansy et al [547,550] used 10% wt/vol egg lecithin in dodecane Cholesterol was added as well We also chose to use 10% egg lecithin ( 60% grade ) in our laboratory Tables 710 and 711 list the results of the various 10% egg lecithin models tested at pION Some of the models were used in conjunction with a sink
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Egg Lecithin 10% wt/vol in Dodecane PAMPA Models, pH 74a
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Sigma (Model 140), No Sink Pe (SD) %R 12 27 (01) 31 (05) 22 (03) 46 (07) 25 71 (30) 53 (05) 41 (16) 39 (07) 44 (07) 40 03 (nd) 84 84 69 84 52 74 50 34 65 78 65 26 2 5 47 6 4 11 8 5 5 8 9 5 80 46 20 7 9 Sigma Sigma 05%Cho (Model 141), (Model 150), Sink No Sink Pe (SD) %R Pe (SD) %R 319 (61) 174 (15) 254 (58) 353 (05) 96 (05) 343 (10) 313 (49) 118 (03) 212 (08) 243 (75) 228 (12) 43 (04) (nd) (nd) (nd) (nd) (nd) (nd) 01 (01) (nd) (nd) 22 (01) (nd) (nd) 423 (27) 214 (14) 138 (121) 09 (02) 20 (01) 41 55 33 35 48 40 18 21 43 49 36 22 9 4 6 4 6 1 4 6 3 3 31 25 20 5 7 07 (03) 31 (03) 18 (12) 13 (04) 40 (07) 38 (03) 38 (02) 44 (04) 35 (03) 27 (03) 44 (08) 55 (02) 37 (01) 01 (01) 002 (003) 69 (39) 10 (01) 03 (01) 03 (01) 0006 (0005) 001 (001) 20 (01) 01 (01) 006 (001) 40 (07) 51 (06) 51 (02) 11 (02) 23 (01) 86 86 83 89 59 75 64 38 70 80 81 65 26 7 3 31 6 4 5 4 4 6 5 0 88 41 23 4 7 Sigma 05%Cho (Model 151), Sink Pe (SD) %R 321 (86) 188 (17) 284 (31) 364 (35) 128 (06) 353 (63) 332 (38) 169 (11) 223 (05) 292 (86) 300 (09) 80 (09) (nd) (nd) (nd) 13 (06) 004 (006) 02 (02) 04 (01) (nd) 009 (004) 22 (01) (nd) (nd) 379 (32) 217 (03) 157 (22) 14 (03) 20 (02) 31 50 23 22 39 34 8 16 34 30 26 12 11 3 3 2 7 2 3 2 4 3 2 33 21 19 3 4
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Ibuprofen (nd) Acetaminophen Naproxen 13 Sulfasalazine 005 Theophylline 02 Ketoprofen 03 (01) Hydrochlorothiazide (nd) Furosemide (nd) Salicyclic acid Piroxicam Sulpiride Terbutaline Progesterone Griseofulvin Carbamazepine Antipyrine Caffeine 21 (01) (nd) (nd) 52 (06) 97 (21) 91 (14) 14 (01) 23 (04)
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condition in the acceptor wells Figure 730 shows permeability and membrane retention results for weak-base probes, using the Sigma-Aldrich source of lecithin, with and without sink and 05% wt/vol cholesterol The presence of a sink dramatically increases permeabilities, as indicated in Figure 730a In some cases, further signi cant increases in permeability were realized by the use of cholesterol, even though its amount was only 05% Only in the diffusion-limited cases (right side of Fig 730a) was there only minimal enhancement due to cholesterol
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10%Egg 10%Egg / SINK in Acceptor 10%Egg + 05% Cho / SINK in Acceptor
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