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20. Parish, W. E.: Immunological tests to predict toxicological hazards to man. 21. Venitt, S.: Microbial tests in carcinogenesis studies. 22. Styles, J. A.: Other short-term tests in carcinogenesis studies. Hayes, A. W., ed. Principles and Methods of Toxicology, 2nd ed. New York: Raven Press. This volume contains the following chapters of particular relevance to this chapter: 6. Chan, P. K., and A. W. Hayes: Principles and methods for acute and eye irritancy. 7. Mosberg, A. T., and A. W. Hayes: Subchronic toxicity testing. 8. Stevens, K. R., and M. A. Gallo: Practical considerations in the conduct of chronic toxicity studies. 9. Roberts, J. F., W. W. Piegorsch, and R. L. Schueler: Methods in testing for carcinogenicity. 10. Zenick, H., and E. D. Clegg: Assessment of male reproductive toxicology: a risk assessment approach. 11. Manson, J. M., and Y. J. Kang: Test methods for assessing female reproductive and developmental toxicology. 12. Kennedy, G. L., Jr: Inhalation toxicology. 13. Patrick, E., and H. I. Maiback: Dermatotoxicology. 14. Brusick, D.: Genetic toxicology. 17. Burger, G. T., and L. C. Miller: Animal care and facilities. 18. Norton, S.: Methods for behavioral toxicology. 26. Dean, J. H., et al.: Immune system; evaluation of injury. 30. Renwick, A. G.: Pharmacokinetics in toxicology. 31. Hogan, M. D., and D. G. Hoel: Extrapolation to humans. Moser, V. C., G. C. Becking, V. Cuomo, et al. The IPCS collaborative study on neurobehavioral screening methods: IV. Control data. Neurotoxicol. 18: 947 967, 1997. Weiss, B., and D. Cory-Slechta. Assessment of behavioral toxicity. In Principles and Methods of Toxicology, 3rd ed. A. Wallace Hayes, ed. New York: Raven Press, 1994.
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Forensic toxicology refers to the use of toxicology for the purposes of the law. It is considered a hybrid of analytical chemistry and fundamental toxicology. The efforts or activities conducted to effectuate this purpose include but are not limited to the following:
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Urine testing to detect drug use Regulatory toxicology Occupational disease Identi cation of causative agents causing death or injury in humans and animals Courtroom testimony and consultation concerning toxicoses
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Analytical toxicology in a clinical setting plays a role similar to that in forensic toxicology. Therefore, this chapter will be divided into forensic and clinical toxicology.
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Until the 1700s convictions associated with homicidal poisoning were based only on circumstantial evidence rather than the identi cation of the actual toxicant within the victim. In 1781, Joseph Plenic stated that the detection and identi cation of the poison in the organs of the deceased was the only true sign of poisoning. Years later, 1813, Mathieiv Or la (considered the father of toxicology) published the rst complete work on the subject of poisons and legal medicine. By 1836, James M. Marsh developed a test for the presence of arsenic in tissue. Then, in 1839, Or la successfully used Marsh s test to identify arsenic extracted from human tissues. Fifty years later, Ernst Wilhelm Heinrich Gutzeit developed a method (Gutzeit test) to quantitate arsenic in tissues. In this process arsenic compounds are reduced by hydrogen produced when zinc and sulfuric acid react. The hydrogen then reduces the arsenic compounds to arsine, which is exposed to paper that has been treated with mercuric chloride solution. This
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A Textbook of Modern Toxicology, Third Edition, edited by Ernest Hodgson ISBN 0-471-26508-X Copyright 2004 John Wiley & Sons, Inc.
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produces a color range from yellow to brown depending on the arsenic concentration. By 1918, the Medical Examiner s Of ce and Toxicology Laboratory was established in New York. The chief forensic toxicologist was Alexander O. Gettler who is considered the father of American toxicology.
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