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INDEX
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Cholesterol esterase, 19, 47 Classes of ions formed by CID, 312 Clearing factor, 251 Cloned enzyme donor immunoassay, 120 Coef cient of variation, 325 Collision-induced dissociation (CID), 303 Competitive inhibitors, 34 Computer search string, 311 Con dence limits, 327 Coomassie Blue, 2, 4, 96 Correlation coef cient, 327 Creatine kinase, 30 assay, 46 Genotype-phenotype linkages, 158 Cross-reactivity, 90, 335 Cutoff values, 344, 335 DABA, see Diaminobenzoic acid method Degree of con dence, 325 Densitometer trace, 183 Density gradients materials, 252 in centrifugation, 252 Diaminobenzoic acid method, 7 dideoxy DNA sequencing method, 241 Diffusion layer, 71, 73, 133 Diphenylamine method, 9 Directed evolution goals, 151 Disc electrophoresis, 175 DNA library, 160 polymerase, 155 probes, 185 Shuf ing, 157 DPA, see Diphenylamine method Dynamic range, 329 Eddy diffusion, 275 Electric eld strength gradient, 178 Electron multiplier, 304 Electron-transfer mediators, 54 Electroosmosis, 173, 227 in silica capillaries, 229 Electropherogram, 233 Electrophoresis activity stains, 183 basic forms, 169 capillary, 227 solid scintillator detector, 239 staining solutions, 181 two-dimensional, 222 Electrophoretic migration theory, 169 mobility, 169 resolution, 177
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Electrostatic analyzer, 301 Enhanced copper, see Lowry method Envelope peak, 305 Enzyme (s) activators, 32 apparent af nity, 35 xed-time assays, 46 immobilized, 71 inhibitors, 33 inhibitory homogeneous immunoassay, 120 kinetics, 21 nomenclature, 17 packed-bed reactors, 79 pH optimum, 72 potentiometric methods, 54 reactors, 61, 77 six main categories, 18 speci c activity, 37, 71 turnover number, 36 Enzyme-linked immunosorbent assays (ELISAs), 112 Enzyme-multiplied immunoassay technique, 118 Enzyme-substrate complex, 21, 37 Epitope, 89 Error-prone PCR, 155 ESI, 299 Ethidium bromide, 9 Evolutionary distance, 154 Evolved subtiligases, 163 Excitation transfer immunoassay, 118 FAB, 297 Family shuf ing, 157 Ferguson plot, 176, 191 Ferricyanide method, 11 Ficoll, 252 Fingerprinting methods, 199 FITC, see Fluorescein-isothiocyanate Flight tube, 302 Fluorescamine, 7 Fluorescein isothiocyanate, 50, 140 Fluorescence polarization, 338 immunoassay, 117 Folin-Ciocalteau reagent, 3 Forensic tests, 200 Fourier-transform ion cyclotron resonance, 303 Free fatty acids, 13 Galactose oxidase, 164 b-Galactosidase, 114, 340 Gel polymerization, 174 Genetic disorder diagnosis, 197 Glucose in serum determination, 44
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INDEX
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Glucose oxidase, 20, 44, 48, 52 immobilized, 81 Glucose-6-phosphate dehydrogenase, 115 Glutaraldehyde, 68 Gradient generators, 253 Guidance documents, 322 Hemoglobin, 217 Hybridomas, 91 I.U., see International unit Immobilized enzyme (s) 61, 74 in thermal biosensors, 145 properties, 71 stability, 75 steric exclusion, 75 Immunoassay (s) chemiluminescent labels, 110 clasi cation, 99 competitive equilibria, 104 cross-reactivity, 122, 125 effective dose, 331 enzyme labels, 112, 118 evaluation of new methods, 121 uorescent labels, 107, 116 hapten heterology, 123 labelling reactions, 101 labels, 99, 128 modern methods, 103 nonspeci c interferences, 125 quantitative, 99 radioisotopic labels, 106 sandwich assays, 104 Immunoblotting, 206 labelling techniques, 207 Immunoelectrophoretic methods, 206 Immunoglobulins, see Antibodies indicator reaction (s), 43-45 Injection electrokinetic, 230 hydrodynamic, 230 Intercalating dyes, 9 Interlaboratory studies, 329, 341 International unit, 36 Intralaboratory studies, 329 Ionizing DNA or RNA, 315 Isoelectric focussing in capillaries, 242 Isoenzymes, 17, 18 identi cation, 196 Isopycnic point, 257 Isotopic peaks, 304, 309 Katal, 37
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Lactate dehydrogenase, 17, 19, 45 Laser-induced uorescence detector, 233 Latex beads, 115 Least-squares regression, 326 Light-scattering, 53 Lineweaver-Burk plot, 26 Lipoproteins, 264 Logit transformation, 106, 275 Log-Logit function, 329 Lowry method, 3, 4 Luciferase, 53 Luminol, 52, 110 MAGIChip, 316 Magnetic sector, 301 MALDI, 299 Manometric methods, 47, 55 Mass lter, see Quadrupole analyzer Mass spectrometry electrophoresis detector, 235 Maxam-Gilbert method, 202 Mean absolute deviation, 325 Michaelis-Menten equation, 24 graphical methods, 25 immobilized enzymes, 79 Microplates, 56 Molar absorptivities, 42, 45, 48 Molecular weight standards, 192 Molybdenum blue, 11 Monoclonal antibodies, 90 Monoisotopic mass, 305 Moving boundary analysis, 260 mRNA display, 160 MS ngerprint, 310 MS/MS instruments, 302 Multiply-charged families of ions, 306 molecular ions, 309 Negative ion mode, 316 Nelson reagents, 11 Nernst layer, see Diffusion layer Neutral lactase, see b-Galactosidase Nick-translation method, 186 Ninhydrin reagent, 6, 43 Nitration method, 6 Nominal mass, 305 Nomograph method, 1 Noncompetitive inhibition, 35 Normal distribution, 325 Normal phase chromatography, 279 Northern blot, 184
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