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1. R. A. W. Johnstone and M. E. Rose, Mass Spectrometry for Chemists and Biochemists, Cambridge University Press, New York, 1996, pp. 34 41. 2. G. Siuzdak, Mass Spectrometry for Biotechnology, Academic Press, New York, 1996, pp. 9 17. 3. G. Siuzdak, Mass Spectrometry for Biotechnology, Academic Press, New York, 1996, pp. 11 18. 4. R. A. W. Johnstone and M. E. Rose, Mass Spectrometry for Chemists and Biochemists, Cambridge University Press, New York, 1996, pp. 34, 105 109. 5. C. N. Mc.Ewen and B. S. Larsen, in Mass Spectrometry of Biological Materials, B. S. Larsen and C. N. McEwen, Eds., Marcel Dekker, New York, 1998, pp. 10 22. 6. C. N. Mc.Ewen and B. S. Larsen, in Mass Spectrometry of Biological Materials, B. S. Larsen and C. N. McEwen, Eds., Marcel Dekker, New York, 1998, pp. 17 19. 7. G. Siuzdak, Mass Spectrometry for Biotechnology, Academic Press, New York, 1996, pp. 35 41. 8. R. A. W. Johnstone and M. E. Rose, Mass Spectrometry for Chemists and Biochemists, Cambridge University Press, New York, 1996, pp. 37, 54. 9. S. L. Mullen and A. G. Maeshall, Anal. Chim. Acta 178, 1985, 17 26. 10. A. P. Snyder, Interpreting Protein Mass Spectra, Oxford University Press, Oxford, UK, 2000, pp. 87 110. 11. M. Mann, R. C. Hendrickson, and A. Pandey. Annu. Rev. Biochem. 70, 2001, 437 473. 12. C. Dass, Principles and Practice of Biological Mass Spectrometry, Wiley-Intercience, New York, 2001, pp. 83 84. 13. G. Siuzdak, Mass Spectrometry for Biotechnology, Academic Press, New York, 1996, pp. 66 68. 14. G. Siuzdak, Mass Spectrometry for Biotechnology, Academic Press, New York, 1996, p. 90. 15. G. Siuzdak, Mass Spectrometry for Biotechnology, Academic Press, New York, 1996, pp. 22 24. 16. C. Dass, Principles and Practice of Biological Mass Spectrometry, Wiley-Interscience, New York, 2001, pp. 125 128. 17. For example, see http://pepsea.protana.com/ or http://www.proteometrics.com/ or http:// prowl.rockefeller.edu/cgi-bin/ProFound. 18. W. J. Grif ths, A. P. Jonsson, S. Liu, D. K. Rai, and Y. Wang, Biochem. J. 355, 2001, 545 561. 19. M. Mann and M. Wilm, Anal. Chem. 66, 1994, 4390 4399. 20. For example, see http://www.mann.embl-heidelberg.de/Services/PeptideSearchIntro. html.
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21. G. Siuzdak, Mass Spectrometry for Biotechnology, Academic Press, New York, 1996, p. 94. 22. A. P. Snyder, Interpreting Protein Mass Spectra, Oxford University Press, Oxford, UK, 2000, pp. 166 167. 23. E. Gustafsson, K. Thoren, T. Larsson, P. Davidsson, K. Karlsson, and C. L. Nilsson, Rapid Commun. Mass Spectrom. 15, 2001, 428 432. 24. B. T. Chait, R. Wang, R. C. Beavis, and S. B. H. Kent. Science 262, 1993, 89 92. 25. T. Keough, T. R. Baker, R. L. Dobson, M. P. Lacey, T. A. Riley, J. A. Hassel eld, and P. E. Hesselberth. Rapid Commun. Mass Spectrom. 7, 1993, 195 200. 26. S. Sauer, D. Lechner, and I. G. Gut, in Mass Spectrometry and Genomic Analysis, J. Nicholas Housby, Ed., Kluwer Academic Publishers, Dordrecht, The Netherlands, 2001, pp. 51 53. 27. N. Potier, A. V. Dorsselaer, Y. Cordier, O. Roch, and R. Bischoff, Nucleic Acid Res. 22, 1994, 3895 3903. 28. S. A. McLuckey, G. J. Van Berkel, and G. L. Glish, J. Am. Soc. Mass Spectrom. 3, 1992, 60 70. 29. J. Ni, S. C. Pomerantz, J. Rozenski, Y. Zhang, and J. A. McCloskey, Anal. Chem. 68, 1996, 1989 1999. 30. P. E. Jackson, M. D. Friesen, and J. D. Groopman, in Mass Spectrometry and Genomic Analysis, J. Nicholas Housby, Ed., Kluwer Academic Publishers, Dordrecht, 2001, pp. 76 92. 31. C. Fenselau and P. A. Demirev, Mass Spectrom. Rev. 20, 2001, 157 171. 32. Z. Wang, L. Russon, L. Li, D. C. Roser, and S. R. Long, Rapid Commun. Mass Spectrom. 12, 1998, 456 464. 33. A. J. Saenz, C. E. Petersen, N. B. Valentine, S. L. Gantt, K. H. Jarman, M. T. Kingsley, and K. L. Wahl, Rapid Commun. Mass Spectrom. 13, 1999, 1580 1585. 34. J. O. Lay, Jr., Mass Spectrom. Rev. 20, 2001, 172 194.
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PROBLEMS 1. Calculate the monoisotopic and average mass (Mr) for the following molecules (Table 15.10).
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TABLE 15.10 Compound Glycine Tryptophan Polipeptide Porcine insulin Myoglobin Big heterodimer protein Formula C2H5NO2 C11H12N2O2 C84H101N15O14 C256H381N65O76S6 C769H1212N210O218S3 C1802H2933N532O462S12 Monoisotopic Average
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