pH 55 pH 60 pH 65 pH 74 in Visual Studio .NET

Creator Data Matrix in Visual Studio .NET pH 55 pH 60 pH 65 pH 74
pH 55 pH 60 pH 65 pH 74
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pH 50
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Compound 41 12 39 42 9 0009 44 19 0005 17 24 01 30 13 29 10 45 10 5 57 10 4 40 10 3 11 10 5 87 10 9 31 10 4 13 10 4 50 10 9 18 10 6 27 10 4 12 10 7 94 10 5 14 10 6 30 56 38 35 20 16 22 21 16 34 25 18 29 14 41 14 41 42 19 003 35 15 0005 06 21 01 30 13 29 58 39 36 24 17 21 20 16 32 23 18 29 14 41 15 42 42 30 009 20 9 0005 02 16 01 30 13
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Pa (10 6 cm/s) Pa (10 6 cm/s) Pa (cm/s) %R 92 10 14 10 4 18 10 3 0013 36 10 5 27 10 8 10 10 4 42 10 5 50 10 9 59 10 7 11 10 4 12 10 7 94 10 5 14 10 6
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Pm (cm/s) 0029 44 10 4 57 10 3 0040 11 10 4 86 10 8 33 10 5 13 10 5 50 10 9 19 10 7 39 10 5 12 10 7 94 10 5 14 10 6
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Pa (10 6 cm/s) Pm (cm/s) Pm (cm/s) %R 32 58 39 37 24 17 20 19 14 24 17 19 29 14
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Pa (10 6 %R cm/s)
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Pa (10 6 cm/s) 41 16 42 42 39 06 4 2 0005 002 4 01 30 13
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Pm (cm/s) 023 33 10 3 0045 032 90 10 4 61 10 7 42 10 6 17 10 6 50 10 9 23 10 8 51 10 6 12 10 7 94 10 5 14 10 6
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%R 34 59 39 39 25 19 13 16 14 19 16 19 29 14
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Verapamil Quinine Propranolol Desipramine Metoprolol Ranitidine Naproxen Ketoprofen Hydrochlorothiazide Furosemide Piroxicam Terbutaline Carbamazepine Antipyrine
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39 7 34 41 3 0003 49 22 0005 42 25 01 30 13
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92 10 14 10 5 18 10 4 13 10 3 36 10 6 27 10 9 27 10 4 38 10 4 50 10 9 56 10 6 49 10 4 12 10 7 94 10 5 14 10 6
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28 55 35 34 19 16 22 21 16 42 28 18 29 14
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PERMEABILITY
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log Po (20%Soy/dodecane: Gradient-pH)
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propranolol
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verapamil
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-2 -4 -6 -8
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hydrochlorothiazide
ketoprofen
carbamazepine
antipyrine
log Po SOY = 264 + 136 log Po DOPC r2 = 097, s = 063, n = 7
(excl metoprolol, quinine, piroxicam)
log Po (2%DOPC/dodecane: Iso-pH)
Figure 744 Collander relationship between intrinsic permeabilities of 20% soy lecithin versus 2% DOPC PAMPA models
octanol water system might suggest that, given a background solution of a lipophilic counterion, ion pair transmembrane transport takes place (eg, Section 45) Such hypotheses can be tested in a direct way in the PAMPA assay If the charged species, especially quaternary ammonium drugs, appear in the acceptor compartment, the case for charged species transport could be further advanced It is very dif cult to make the case for charged-species transmembrane transport using the in vitro cultured cell model, because of the simultaneous presence of several possible transport mechanisms [1] 7771 The Case for Charged-Species Transport from Cellular and Liposomal Models Trimethylaminodiphenylhexatriene chloride (TMADPH; Fig 745) is a uorescent quaternary ammonium molecule that appears to permeate cell membranes [595] TMADPH uoresces only when it is in the bilayer, and not when it is dissolved in water Therefore, its location in cells can be readily followed with an imaging uorescence microscope One second after TMADPH is added to the extracellular solution bathing HeLa cell types, the charged molecule fully equilibrates between the external buffer and the extracellular (outer) lea et bilayer Washing the cells for one minute removes >95% of the TMADPH from the outer lea et If the cells are equilibrated with TMADPH for 10 min at 37 C, followed by a one-minute wash that removed the TMADPH from the outer lea et, the uorescent molecule is